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Contents

Purdue University's Biological Electron Microscopy Facility

This page is for current users of the facility, and contains laboratory protocols and procedures, and other information relevant to the operation of our equipment. For a general description and scope of the facility, please visit our web page at (BEMF).

Rates

Media:CurrentRates.pdf

Scheduling

(CORAL)

Protocols and Procedures:

Media:OsO4waste_protocol.pdf

Media:Osmium_MSDS.pdf

Media:CarbonFilms.pdf

Media:Darkroom.pdf NEW!

Media:P2O5.pdf

Media:DryPumpingStation.pdf

Media:EMITECH_K950_Evaporator.pdf‎

Media:EMITECHGlowDischarge.pdf

Media:CM200 Procedures.pdf

Equipment Condition and updates:

Microscopes:

FEI Titan Krios: Leginon installed. Calibrations in progress. Some testing done and successful.

FEI CM200F: No film usage possible at the moment due to air compressor problem affecting camera chamber vent/pump operation.

FEI CM300. Currently unavailable.


Ancillary equipment:

FEI Vitrobot,

Gatan CP3,

Manual freeze-plungers (2),

Emitech evaporator (currently only good for glow discharge),

Ladd evaporator,

Gatan dry pumping stations (2 available, one unavailable),

Gatan cryoholders:

No. 1. unavailable,

No. 2. OK,

No. 3. OK,

No. 4. broken pin,

No. 5. vibration problem,

Electron Microscope Advisory Committee:

Michael Rossmann, Wen Jiang, Jason Lanman, Richard Kuhn, David Thompson, Chengde Mao, Lia Stanciu, Agustin Avila-Sakar. The facility was granted CTSI core designation in February 2013.

Staff:

Christopher Gilpin, Interim Director (765)494-1487, (765) 494 7750

Valorie Bowman, Senior Research Electron Microscopist (765)494-5643

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